IMMOBILIZATION OF LIPASE BY PHYSICAL ADSORPTION ON POLYETHYLENE TEREPHTHALATE BEADS
Hristina N. Panajotova, Valentina T. Dobreva, Hristina N. Strinska, Boriana Y. Zhekova and Georgi T. Dobrev
Pages: 45-51
ABSTRACT An extracellular lipase of Aspergillus carbonarius was immobilized by adsorption onto a polyethylene terephthalate support. The optimum conditions for immobilization of lipase were studied. The optimum concentration of enzyme solution for immobilization was 35 kU/g of support, at which 2.28% immobilization efficiency was achieved. The optimum incubation time for immobilization of the enzyme was 4 hours. The optimum pH for immobilization was pH 5. The immobilized lipase retained 93% of its initial activity after 5 cycles of transesterification reaction in n-hexane medium.
Keywords: adsoption, Aspergillus carbonarius, immobilization, lipase, polyethylene terephthalate