IMMOBILIZATION OF LIPASE BY PHYSICAL ADSORPTION ON POLYETHYLENE TEREPHTHALATE BEADS
Hristina N. Panajotova, Valentina T. Dobreva, Hristina N. Strinska, Boriana Y. Zhekova and Georgi T. Dobrev
ABSTRACT An extracellular lipase of Aspergillus carbonarius was immobilized by adsorption onto a polyethylene terephthalate support. The optimum conditions for immobilization of lipase were studied. The optimum concentration of enzyme solution for immobilization was 35 kU/g of support, at which 2.28% immobilization efficiency was achieved. The optimum incubation time for immobilization of the enzyme was 4 hours. The optimum pH for immobilization was pH 5. The immobilized lipase retained 93% of its initial activity after 5 cycles of transesterification reaction in n-hexane medium.
Keywords: adsoption, Aspergillus carbonarius, immobilization, lipase, polyethylene terephthalate